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RAPD typing (random amplification of polymorphic DNA)
RAPD typing (random amplification of polymorphic DNA) RAPD typing (random amplification of polymorphic DNA)
Agarose gel with RAPD profiles. DNA appears as distinct bright white bands under UV light.

RAPD is a PCR based typing method. DNA is amplified by PCR from the target organism at relatively low stringency using one or more short primers . This results in the amplification of a number of ”random” fragments. The amplified fragments are separated by agarose gel electrophoresis and patterns hare compared.

Lane 1 is a molecular size marker. Lanes 2 to 9 are RAPD patterns of strains that were typed. The strains in lanes 2 and 3 have an identical pattern and the strain in lanes 4 and 5 have show the same pattern, which is almost identical to the pattern in lanes 2 and 3.

© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen
Denmark