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The purpose of this test is to determine whether an organism can produce acetylmethylcarbinol (acetoin) from fermentation of glucose. The culture is incubated in Clark and Lubb's medium. Half of this medium is then used for the methyl red test and the other half for a Voges-Proskauer test. Alpha-naphtol (5%) and potassium hydroxide (40%) are added to the the medium. If acetoin is present the medium will turn pink-reddish in colour.
1. Uninoculated medium.
2. Positive reaction: Klebsiella.
3. Negative reaction: Escherichia coli.
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© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen
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