Hippurate Hydrolysis
This test is used to determine whether a micro-organism, by action of the enzyme hippurate hydrolase, can hydrolyse sodium hippurate to benzoic acid and glycine. After inoculation the medium containing 1% sodium hippurate in distilled water is incubated at 37°C for 2 hours. Ninhydrin solution is then added to the tube and it is incubated for a further 10 minutes. This test does not require the micro-organisms to grow, but instead it detects the presence of already formed enzyme by testing for one of the end products of the hydrolysis, namely glycine. If glycine is present a blue coloured complex is formed. 1. Positive reaction: Campylobacter jejuni 2. Negative reaction: Campylobacter coli
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