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Gelatin Hydrolysis
Gelatin Hydrolysis Gelatin Hydrolysis
Aeromonas hydrophila on gelatin agar prior to flooding with sulphosalicylic acid.
Positive reaction: The same plate after flooding with sulphosalicylic acid.
This test is used to determine whether an organism can hydrolyse gelatin by action of gelatinase. After incubation, the plates are flooded with sulphosalicylic acid. A clear zone around and under the streak indicates that hydrolysis of the gelatin has taken place.

The pictures below show an example of a negative reaction.

Click on the pictures to see enlarged versions.
© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen