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Aeromonas hydrophila on gelatin agar prior to flooding with sulphosalicylic acid. |
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Positive reaction: The same plate after flooding with sulphosalicylic acid. |
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This test is used to determine whether an organism can hydrolyse gelatin by action of gelatinase. After incubation, the plates are flooded with sulphosalicylic acid. A clear zone around and under the streak indicates that hydrolysis of the gelatin has taken place.
The pictures below show an example of a negative reaction.
Click on the pictures to see enlarged versions. |
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