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Carbohydrate Fermentation 2
The purpose of this test is to determine whether an organism can ferment a specific carbohydrate with production of acid. In this example lactose is used. The indicator is bromthymol blue and turns yellow when pH is low.

1. Uninoculated medium.

2. Positive reaction: Escherichia coli.

3. Negative reaction: Salmonella. The medium is lighter in colour than the uninoculated medium due to turbidity caused by the growth of bacteria.





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© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen
Denmark