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Clostridium perfringens
Clostridium perfringens Clostridium perfringens
Clostridium perfringens grown anaerobically on blood agar at 37 ° C for one day. Cl. perfringens is the obligate anaerobic, but one of the clostridial that can grow at a relatively high value of the Eh.
Clostridium perfringens is large box-shaped rods. There is normally no spores, since the formation of these require alkaline conditions.
© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen
Denmark

Clostridium perfringens - Plate Count Agar
Clostridium perfringens Clostridium perfringens Clostridium perfringens
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Click the pictures to enlarge

There is prepared a dilution series of Clostridium perfringens plated in PCA and incubated aerobically at 25° C for three days. As the incubation is carried out aerobically, some of the colonies growing on the surface. It is the large off-white colonies. Colonies which lies between the agar and the petri dish is smaller and more transparent. Colonies which is located in the agar is lenticular.
© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen
Denmark

Clostridium perfringens - Plate Count Agar closeup
Clostridium perfringens
10-4  
The round whitish colonies are located on the surface. The lenticular colonies located in the agar and the round gray, transparent colonies are located between the Petri dish and the agar.
© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen
Denmark